8FIN

Multi-state design of two-state switchable hinge proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	22.860, 81.735, 154.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.660 - 2.300
R-factor	0.2421
Rwork	0.239
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.290
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.660	2.390
High resolution limit [Å]	2.300	2.300
Rmerge	0.115	0.597
Rpim	0.032	0.157
Number of reflections	13723	1431
<I/σ(I)>	15.19	4.77
Completeness [%]	99.8	99.93
Redundancy	13.7	14.9
CC(1/2)	1.000	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M SPG buffer pH 7, 25% (w/v) PEG 1500