8FHX
Structure of Lettuce aptamer bound to DFHBI-1T
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-26 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.912 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.015, 44.417, 118.921 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.610 - 2.500 |
R-factor | 0.2297 |
Rwork | 0.225 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8fhv |
RMSD bond length | 0.005 |
RMSD bond angle | 0.775 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.460 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.102 | 0.287 |
Number of reflections | 4870 | 473 |
<I/σ(I)> | 13.9 | 2 |
Completeness [%] | 97.6 | |
Redundancy | 10.2 | |
CC(1/2) | 0.998 | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.05 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 30% (v/v) polyethylene glycol monoethyl ether 550 |