8FHB
Protein 32 with aldehyde deformylating oxidase activity from Synechococcus sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2022-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.192, 74.330, 111.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.165 - 1.950 |
| Rwork | 0.169 |
| R-free | 0.21670 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.117 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.320 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.059 | 0.666 |
| Rmeas | 0.061 | 0.692 |
| Rpim | 0.017 | 0.186 |
| Number of reflections | 17168 | 1177 |
| <I/σ(I)> | 26.7 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.2 | 13.7 |
| CC(1/2) | 1.000 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG 4,000, 200 mM MgCl2, 100 mM HEPES HCl |
