8FG7

Apo mouse acidic mammalian chitinase, catalytic domain at 277 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2022-10-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.11580
Spacegroup name	P 21 21 21
Unit cell lengths	63.647, 71.844, 84.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.780 - 1.640
R-factor	0.1481
Rwork	0.147
R-free	0.17130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	xia2 (0.3.8.0)
Data scaling software	DIALS (2.2.10-g6dafd9427-release)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.780	1.699
High resolution limit [Å]	1.640	1.640
Rmerge	0.249
Rmeas	0.259
Rpim	0.071
Number of reflections	47999	4688
<I/σ(I)>	7	1.19
Completeness [%]	99.4	98.65
Redundancy	12.9	13.2
CC(1/2)	0.996	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	11 mg/mL AMCase catalytic domain 19.8% PEG 3350 0.198 M Magnesium Formate 0.01 M Phenol