8FG6
Design of amyloidogenic peptide traps
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.540, 64.540, 112.618 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.890 - 2.300 |
| R-factor | 0.21463 |
| Rwork | 0.212 |
| R-free | 0.25952 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.628 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.890 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.118 | 0.962 |
| Rpim | 0.033 | 0.261 |
| Number of reflections | 12554 | 1224 |
| <I/σ(I)> | 12.97 | 2.87 |
| Completeness [%] | 99.5 | |
| Redundancy | 13.6 | 14.5 |
| CC(1/2) | 0.996 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 0.1 M Tris pH 7.8, poly-gamma-glutamic acid, 15% PEG 4000 |
