8FFC
Crystal structure of iron bound Dps protein (PA0962) from Pseudomonas aeruginosa (cubic form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 291 |
Detector technology | PIXEL |
Collection date | 2020-10-07 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 3 |
Unit cell lengths | 223.900, 223.900, 223.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.910 - 1.850 |
R-factor | 0.1633 |
Rwork | 0.162 |
R-free | 0.19190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.989 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19rc5_4043) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.860 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.149 | 1.985 |
Rmeas | 0.153 | 2.035 |
Rpim | 0.034 | 0.444 |
Total number of observations | 6542757 | 325945 |
Number of reflections | 315151 | 15535 |
<I/σ(I)> | 15.2 | 1.9 |
Completeness [%] | 100.0 | |
Redundancy | 20.8 | 21 |
CC(1/2) | 0.999 | 0.684 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 8% (w/v) PEG 8000, 100 mM Hepes. Soaking: crystals were transferred to a solution containing 80% crystallant, 20% PEG 200 and 50 mM FeCl2 and soaked for 10 minutes. Samples were harvested directly from the soaking solution |