8FFA

Crystal structure of Apo Dps protein (PA0962) from Pseudomonas aeruginosa (cubic form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	291
Detector technology	PIXEL
Collection date	2020-06-01
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	1.0000
Spacegroup name	P 21 3
Unit cell lengths	223.465, 223.465, 223.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.970 - 2.150
R-factor	0.1852
Rwork	0.183
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc5_4043)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.970	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.202	2.343
Rmeas	0.207	2.400
Rpim	0.045	0.518
Total number of observations	4163876	210869
Number of reflections	200207	9858
<I/σ(I)>	12.5	1.7
Completeness [%]	100.0
Redundancy	20.8	21.4
CC(1/2)	0.999	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1.1 M sodium malonate, 100 mM Hepes, 0.5% (v/v) Jeffamine ED-2001