8FEU

Flavanone 4-Reductase from Sorghum bicolor-NADP(H) and naringenin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-13
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9774
Spacegroup name	I 41 2 2
Unit cell lengths	165.180, 165.180, 207.655
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.440 - 2.120
R-factor	0.1995
Rwork	0.199
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.440	2.196
High resolution limit [Å]	2.120	2.120
Number of reflections	80982	15966
<I/σ(I)>	17.62
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.999	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M HEPES, pH 7.5, 2 M Ammonium Sulfate and 2 % PEG 400