8FER

16mer self-complementary duplex RNA with two continuous s(2)U:s(2)U pairs

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.037690
Spacegroup name	H 3 2
Unit cell lengths	43.810, 43.810, 258.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.065 - 1.540
Rwork	0.219
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.757
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.570
High resolution limit [Å]	1.540	1.540
Rmerge	0.113	0.429
Rmeas	0.121	0.461
Rpim	0.043	0.166
Number of reflections	14744	747
<I/σ(I)>	13.7	4.4
Completeness [%]	99.7	100
Redundancy	7.9	7.6
CC(1/2)	0.986	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.02 M Magnesium chloride hexahydrate, 0.002 M Cobalt(II) chloride hexahydrate, 0.05 M HEPES sodium pH 7.5, 2.0 M Ammonium sulfate, 0.001 M Spermine