8FEP
16mer self-complementary duplex RNA with two continuous native U:U pairs
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033175 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 41.717, 41.717, 126.234 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.048 - 1.550 |
| Rwork | 0.206 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.548 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.050 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.066 | 0.187 |
| Rmeas | 0.070 | 0.199 |
| Rpim | 0.025 | 0.066 |
| Number of reflections | 6354 | 310 |
| <I/σ(I)> | 30 | 2.1 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 8.8 | 9 |
| CC(1/2) | 0.999 | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2 M Lithium sulfate, 3 % w/v 2-Methyl-2,4-pentanediol |
