8FDP

The native structure of a dodecamer: 5'-CGCAAATTTGCG-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	24.977, 41.507, 65.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.340 - 1.880
R-factor	0.2491
Rwork	0.246
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.248
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.340	1.950
High resolution limit [Å]	1.880	1.880
Rmerge	0.010
Number of reflections	5921	577
<I/σ(I)>	25
Completeness [%]	99.5
Redundancy	2
CC(1/2)	1.000	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	MPD, SODIUM CACODYLATE TRIHYDRATE, SPERMINE TETRACHLORIDE, SODIUM CHLROIDE, MAGNESIUM HEXAHYDRATE