8FDO

SARS-CoV-2 fusion peptide epitope scaffold FP15 bound to DH1058

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	48.390, 50.580, 146.240
Unit cell angles	90.00, 96.89, 90.00

Refinement procedure

Resolution	48.390 - 2.200
R-factor	0.1911
Rwork	0.189
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tow 5yo4
RMSD bond length	0.006
RMSD bond angle	0.834
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev-3758-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.390	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	35552	3518
<I/σ(I)>	23.64	2.26
Completeness [%]	98.2
Redundancy	7.5
CC(1/2)	0.501	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Bis-Tris, 25% PEG 3350