8FCF
Crystal structure of PLVAP CC1 in I212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 33.771, 88.999, 180.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.930 - 1.950 |
| R-factor | 0.246 |
| Rwork | 0.245 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.928 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rpim | 0.041 | 0.329 |
| Number of reflections | 14322 | 753 |
| <I/σ(I)> | 8.7 | 2.8 |
| Completeness [%] | 92.0 | 88 |
| Redundancy | 12.1 | |
| CC(1/2) | 0.995 | 0.807 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate, pH 5.0 and 15% PEG 4000 |
