8FCF

Crystal structure of PLVAP CC1 in I212121 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	I 21 21 21
Unit cell lengths	33.771, 88.999, 180.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.930 - 1.950
R-factor	0.246
Rwork	0.245
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.050
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.928	2.050
High resolution limit [Å]	1.950	1.950
Rpim	0.041	0.329
Number of reflections	14322	753
<I/σ(I)>	8.7	2.8
Completeness [%]	92.0	88
Redundancy	12.1
CC(1/2)	0.995	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate, pH 5.0 and 15% PEG 4000