8FC3

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and telithromycin at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.120, 449.230, 619.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	151.990 - 2.600
R-factor	0.2035
Rwork	0.201
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhx
RMSD bond length	0.004
RMSD bond angle	0.882
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	151.990	151.990	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.239	0.053	2.219
Rmeas	0.262	0.058	2.427
Total number of observations	10689294
Number of reflections	1761733	20596	129859
<I/σ(I)>	7.36	27.98	0.93
Completeness [%]	100.0	99	100
Redundancy	6.067	6.086	6.169
CC(1/2)	0.990	0.997	0.187

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME