8FBW
Crystal structure of SIV-1 V2 antibody NCI05 in complex with a V2 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 130.650, 130.650, 216.948 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.910 - 2.350 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.554 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487-000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 10.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.119 | 0.044 | 1.060 |
| Number of reflections | 46240 | 4344 | 2275 |
| <I/σ(I)> | 24.6 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 22 | 11.5 | 22.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.2 M sodium chloride, 28.5% PEG8000, 0.1 M phosphate/citrate, pH 4.2 |
