8FB7
Crystal structure of Ky15.10 Fab in complex with circumsporozoite protein NPDP peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 200.432, 67.945, 83.233 |
| Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
| Resolution | 29.270 - 1.750 |
| R-factor | 0.1637 |
| Rwork | 0.163 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 108854 | 5402 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M calcium chloride, 20% PEG3350 |
