8FAS

Crystal structure of Ky230 Fab in complex with circumsporozoite protein NANP5 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	54.237, 61.352, 135.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.920 - 1.550
R-factor	0.1662
Rwork	0.165
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.232
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.570
High resolution limit [Å]	1.550	1.550
Number of reflections	66528	2442
<I/σ(I)>	16
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.998	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M di-ammonium tartrate, 20% PEG3350