8FAQ

Structure of Hemagglutinin from Influenza A/Victoria/22/2020

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-03
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12723
Spacegroup name	H 3 2
Unit cell lengths	99.705, 99.705, 395.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	79.252 - 2.035
Rwork	0.203
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yp4
RMSD bond length	0.007
RMSD bond angle	1.473
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.252	2.071
High resolution limit [Å]	2.035	2.035
Rmerge	0.077	1.642
Rmeas	0.079
Number of reflections	48446	2360
<I/σ(I)>	21.6	2.2
Completeness [%]	98.6	98
Redundancy	20.4
CC(1/2)	0.999	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291.15	50% PEG-200, 0.1M citrate pH 5.5, final pH 6.9