8FAM
Edited Octopus bimaculoides Synaptotagmin 1 C2A (I248V) at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2019-11-08 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.924, 49.422, 71.706 |
| Unit cell angles | 90.00, 106.28, 90.00 |
Refinement procedure
| Resolution | 41.510 - 1.950 |
| R-factor | 0.1795 |
| Rwork | 0.176 |
| R-free | 0.21280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.824 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487-000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Rmerge | 0.127 | 0.034 | 0.801 |
| Total number of observations | 64770 | ||
| Number of reflections | 35320 | 2128 | 682 |
| <I/σ(I)> | 4.6 | ||
| Completeness [%] | 83.6 | 99.7 | 32 |
| Redundancy | 1.8 | 1.9 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 23% PEG 3350, 100 mM CHES |
