8FAF
Unedited Octopus bimaculoides Synaptotagmin 1 C2A at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2019-11-08 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.728, 54.940, 84.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.070 - 1.850 |
| R-factor | 0.185 |
| Rwork | 0.181 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.809 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20,1-4487-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.070 | 2.020 |
| High resolution limit [Å] | 1.850 | 1.950 |
| Rmerge | 0.620 | |
| Number of reflections | 20317 | 2009 |
| <I/σ(I)> | 7.1 | 0.9 |
| Completeness [%] | 83.0 | 41.26 |
| Redundancy | 3.2 | 1.6 |
| CC(1/2) | 0.995 | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 23% PEG 3350, 100 mM CHES |
