8FA4
Crystal structure of Xanthomonas campestris GanA beta-galactosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 158.132, 158.132, 94.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.074 - 1.699 |
| R-factor | 0.1614 |
| Rwork | 0.161 |
| R-free | 0.19450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.326 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 1.730 |
| High resolution limit [Å] | 1.699 | 1.700 |
| Rmerge | 0.185 | 1.506 |
| Rmeas | 0.191 | 1.555 |
| Rpim | 0.047 | 0.384 |
| Total number of observations | 102559 | |
| Number of reflections | 131695 | 6396 |
| <I/σ(I)> | 14.1 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 16.3 | 16 |
| CC(1/2) | 0.998 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M trilithium citrate, 25% PEG 3350 |
