Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 142.971, 142.971, 75.135 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.720 - 2.600 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.720 | 39.720 | 2.720 |
| High resolution limit [Å] | 2.600 | 9.010 | 2.600 |
| Rmerge | 0.195 | 0.032 | 9.900 |
| Rmeas | 0.196 | 0.033 | 15.261 |
| Rpim | 0.022 | 0.004 | 1.682 |
| Total number of observations | 1128236 | ||
| Number of reflections | 14420 | 415 | 1708 |
| <I/σ(I)> | 29.5 | ||
| Completeness [%] | 100.0 | 99 | 100 |
| Redundancy | 78.2 | 58.9 | 81.3 |
| CC(1/2) | 1.000 | 1.000 | 0.365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20 to 30 % PEG 2000 MME, 0.2 M (NH4)2SO4, 0.1 M HEPES |
