8F9W

Crystal structure of Ky15.8 Fab in complex with circumsporozoite protein NPDP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 1 21 1
Unit cell lengths	78.672, 130.851, 101.540
Unit cell angles	90.00, 109.34, 90.00

Refinement procedure

Resolution	39.270 - 2.400
R-factor	0.2028
Rwork	0.202
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.533
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	75328	3622
<I/σ(I)>	11.2
Completeness [%]	99.3
Redundancy	6.9
CC(1/2)	0.997	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M ammonium sulfate, 0.1 M HEPES pH 7.5, 25% PEG3350