8F9V
Crystal structure of Ky15.8 Fab in complex with circumsporozoite protein KQPA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033167 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.046, 133.488, 101.861 |
Unit cell angles | 90.00, 110.15, 90.00 |
Refinement procedure
Resolution | 39.310 - 2.250 |
R-factor | 0.2592 |
Rwork | 0.259 |
R-free | 0.27890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.719 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.280 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 90017 | 3503 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 94.5 | |
Redundancy | 2.7 | |
CC(1/2) | 0.676 | 0.716 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% isopropanol, 20% PEG4000, 0.1 M tri-sodium citrate pH 5.6 |