8F9U

Crystal structure of Ky15.7 Fab in complex with circumsporozoite protein NPDP peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 21 21 21
Unit cell lengths	50.990, 60.696, 147.834
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.260 - 1.700
R-factor	0.2089
Rwork	0.208
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.617
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	50113	2481
<I/σ(I)>	10.3
Completeness [%]	97.4
Redundancy	5.5
CC(1/2)	0.833	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M di-ammonium hydrogen phosphate, 20% PEG3350