8F95

Crystal structure of Ky15.2 Fab in complex with circumsporozoite protein DND peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033192
Spacegroup name	P 21 21 21
Unit cell lengths	75.584, 90.514, 133.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.830 - 2.450
R-factor	0.1895
Rwork	0.187
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.598
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.450	2.450
Number of reflections	34421	1586
<I/σ(I)>	8.9
Completeness [%]	99.9
Redundancy	13.4
CC(1/2)	0.995	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	25% (w/v) PEG3350, 0.2 M NaCl, 0.1 M Tris pH 8.5