8F90
Intramolecular ester bond-containing repeat domain 2 from Suipraoptans intestinalis adhesin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953739 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.075, 90.603, 89.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.301 - 1.600 |
| Rwork | 0.157 |
| R-free | 0.19810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.279 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.301 | 1.490 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 114397 | 5159 |
| <I/σ(I)> | 10.6 | 1 |
| Completeness [%] | 99.6 | 92.2 |
| Redundancy | 13.6 | 12.1 |
| CC(1/2) | 0.999 | 0.211 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M MgCl2, 0.1 M Bis-Tris, 25% PEG 3350 |
