8F8X

Crystal structure of Nb.X0 bound to the afucosylated human IgG1 fragment crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	C 1 2 1
Unit cell lengths	125.577, 92.233, 76.497
Unit cell angles	90.00, 117.04, 90.00

Refinement procedure

Resolution	46.120 - 2.600
R-factor	0.2214
Rwork	0.215
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.790
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rpim	0.050	0.574
Number of reflections	21352	1051
<I/σ(I)>	23.6
Completeness [%]	90.7
Redundancy	2.5
CC(1/2)	0.996	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 % w/v PEG 6000, 0.1 M Sodium citrate 4.0, 0.2 M Lithium chloride. No additional cryoprotectant.