8F8N

Crystal structure of the Arabidopsis SPIRAL2 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792603
Spacegroup name	P 21 21 21
Unit cell lengths	35.480, 47.732, 111.113
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.799 - 1.798
R-factor	0.189
Rwork	0.186
R-free	0.21710
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.323
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.798	3.880	1.800
Rmerge	0.098	0.050	0.194
Number of reflections	33280	3249	3158
<I/σ(I)>	12.7
Completeness [%]	98.0	95.1	92.1
Redundancy	5.6	5.6	3.2
CC(1/2)	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	Well solution (1ml) = 1.05 M Ammonium sulfate, 100 mM sodium acetate pH 4.6 Protein solution = 7 mg/ml protein in 25 mM Tris pH 8.5, 500 mM NaCl, and 0.1% beta-mercaptoethanol, 5 mM L-methionine Hanging drop = 2+2 microliters