8F7Z

VRC34.01_mm28 bound to fusion peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	129.850, 130.554, 130.589
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.220 - 2.700
R-factor	0.2116
Rwork	0.210
R-free	0.24280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold model
RMSD bond length	0.006
RMSD bond angle	0.973
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.134	0.581
Rmeas	0.146	0.629
Rpim	0.057	0.238
Number of reflections	60434	3015
<I/σ(I)>	16	2.6
Completeness [%]	97.8
Redundancy	6.5
CC(1/2)	0.991	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG 3350, 1.4M Na/K Phosphate pH 7.5