8F7N

Crystal structure of chemoreceptor McpZ ligand sensing domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2021-07-10
Detector	RDI CMOS_8M
Wavelength(s)	1
Spacegroup name	I 41 2 2
Unit cell lengths	184.330, 184.330, 113.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.600 - 2.700
R-factor	0.2452
Rwork	0.244
R-free	0.25960
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.206
Data reduction software	iMOSFLM (v7.4.0)
Refinement software	PHENIX (v1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	2.650
High resolution limit [Å]	2.540	2.540
Rmerge	0.166
Rmeas	0.169
Rpim	0.031
Number of reflections	32346	3873
<I/σ(I)>	17.8	0.5
Completeness [%]	99.9	100
Redundancy	20
CC(1/2)	1.000	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	Lanthanides, MOPSO, Bis-Tris pH 6.5, and Precipitant Mix 8 (PEG 20000, Trimethylpropane, NDSB 195)