8F72
Phage P32 gp64- RNA polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-06 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.330, 106.320, 112.360 |
| Unit cell angles | 90.00, 102.26, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.20761 |
| Rwork | 0.205 |
| R-free | 0.25746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.337 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.007 | 0.731 |
| Number of reflections | 281832 | 6873 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.998 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 273 | 20-30% PEG 4000 100 mM KCl HEPES pH 7.5 |
