8F71
Crystal structure of the histidine kinase domain of bacteriophytochrome RpBphP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 84.851, 84.851, 346.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.110 - 3.190 |
| R-factor | 0.2238 |
| Rwork | 0.222 |
| R-free | 0.26430 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.190 | 3.200 |
| Rpim | 0.041 | 0.429 |
| Number of reflections | 22330 | 645 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 8.6 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% PEG3350, 0.2 M sodium chloride, 0.1 M Tris-HCl, pH 8.5 |
