8F70

Identification of an Immunodominant region on a Group A Streptococcus T-antigen Reveals Temperature-Dependent Motion in Pili

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	110
Detector technology	PIXEL
Collection date	2018-07-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95374
Spacegroup name	I 1 2 1
Unit cell lengths	34.294, 46.976, 380.633
Unit cell angles	90.00, 90.12, 90.00

Refinement procedure

Resolution	47.580 - 2.290
R-factor	0.19261
Rwork	0.189
R-free	0.25366
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6n0a
RMSD bond length	0.007
RMSD bond angle	1.489
Data reduction software	XDS (VERSION Jun 1, 2017)
Data scaling software	Aimless (1.17.29)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.580	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.154	1.090
Rmeas	0.170	1.206
Rpim	0.071	0.506
Total number of observations	145410	13215
Number of reflections	27529	2540
<I/σ(I)>	7.2	1.7
Completeness [%]	99.4
Redundancy	5.3	5.2
CC(1/2)	0.993	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.12M 1,6-Hexanediol, 0.12M 1-Butanol, 0.12M 1,2-Propanediol, 0.12M 2-Propanol, 0.12M 1,4-Butanediol, 0.12M 1,3-Propanediol, 0.1M Sodium HEPES, 0.1M MOPS (acid), 20% v/v Ethylene glycol, 10 % w/v PEG 8000