8F6S

LSD1-CoREST in complex with T105

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-06
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.96546
Spacegroup name	I 2 2 2
Unit cell lengths	119.016, 179.591, 232.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.680 - 2.910
R-factor	0.2155
Rwork	0.214
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v1d
RMSD bond length	0.012
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.410	2.990
High resolution limit [Å]	2.910	2.910
Rmerge	0.154	2.667
Rmeas	0.174	3.027
Rpim	0.079	1.401
Total number of observations	243688	17323
Number of reflections	54540	3964
<I/σ(I)>	5.6	0.6
Completeness [%]	98.8
Redundancy	4.5	4.4
CC(1/2)	0.995	0.147

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.2 Na/K Tartrate, 100 mM ADA pH 6.5