8F6O
anti-BTLA monoclonal antibody h22B3 in complex with BTLA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 68.950, 88.060, 233.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.000 - 2.310 |
| R-factor | 0.2177 |
| Rwork | 0.215 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aw2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (v2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.430 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.093 | 0.505 |
| Number of reflections | 31602 | 4567 |
| <I/σ(I)> | 11.9 | 3.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 23% PEG 10K + 200mM Magnesium Formate + 100mM Sodium Acetate pH 5.4 |
