8F5T
Rabbit muscle pyruvate kinase in complex with sodium and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 142.453, 113.759, 169.144 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 47.160 - 2.410 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g50 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.090 | 0.041 | 0.478 |
| Total number of observations | 870606 | ||
| Number of reflections | 397612 | 40558 | 38556 |
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 97.4 | 99.4 | 94.3 |
| Redundancy | 2.2 | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 50 mM Tris 50 mM NaCl and a range of 7.5 to 10% PEG 20,000 |
