8F5Q
Crystal structure of human PCNA in complex with the PIP box of FBH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2022-03-17 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.28 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 81.950, 82.210, 118.558 |
| Unit cell angles | 90.00, 91.26, 90.00 |
Refinement procedure
| Resolution | 41.790 - 1.900 |
| R-factor | 0.2254 |
| Rwork | 0.224 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iy4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.260 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.790 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | |
| Number of reflections | 62010 | 11431 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 98.3 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.997 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES, pH 7.5, 0.2 M magnesium chloride, 30% PEG400 |
