8F5H

SARS-CoV-2 S2 helix epitope scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-09-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	175.620, 28.660, 65.500
Unit cell angles	90.00, 106.66, 90.00

Refinement procedure

Resolution	44.550 - 2.300
Rwork	0.254
R-free	0.27450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n1b
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev-3758-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.550	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	13794	1344
<I/σ(I)>	16.14	0.613
Completeness [%]	95.1	92.61
Redundancy	13.5
CC(1/2)	0.765	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M citric acid pH 3.5, 3.0M NaCl