8F5C
Mirror-image DNA containing 2'-OMe-L-dC modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2022-10-15 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9787 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 41.900, 41.900, 23.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.710 - 1.150 |
R-factor | 0.19318 |
Rwork | 0.193 |
R-free | 0.20362 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7moo |
RMSD bond length | 0.037 |
RMSD bond angle | 3.357 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.820 | 1.180 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.040 | 0.723 |
Rmeas | 0.043 | 0.795 |
Rpim | 0.016 | 0.325 |
Number of reflections | 7947 | 558 |
<I/σ(I)> | 28.1 | 3 |
Completeness [%] | 99.8 | 97.4 |
Redundancy | 13 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Magnesium chloride, 0.1 M HEPES sodium pH 7.5, 20% v/v (+/-)-2-Methyl-2,4-pentanediol |