8F48
Human CASQ1 mutant - S248E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2019-03-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.650, 107.340, 108.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.300 - 2.900 |
| R-factor | 0.2248 |
| Rwork | 0.218 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5crd |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.488 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 16929 | 1637 |
| <I/σ(I)> | 9.39 | |
| Completeness [%] | 99.5 | |
| Redundancy | 2 | |
| CC(1/2) | 0.996 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 293 | 0.1M sodium acetate, 8% PEG 400, pH 4.1 |
