8F43
HNH Nuclease Domain from G. stearothermophilus Cas9, K597A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-18 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.920105 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 45.415, 85.751, 26.921 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.920 - 1.370 |
Rwork | 0.168 |
R-free | 0.18410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7mpz |
RMSD bond length | 0.005 |
RMSD bond angle | 0.913 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.580 | 1.390 |
High resolution limit [Å] | 1.370 | 1.370 |
Number of reflections | 22933 | 1146 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 100.0 | |
Redundancy | 13.1 | |
CC(1/2) | 0.988 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M calcium chloride hexahydrate, 0.1M HEPES pH 7.0, 20% polyethylene glycol 6000 |