8F2Z

LSD1-CoREST in complex with AW2, short soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999869
Spacegroup name	I 2 2 2
Unit cell lengths	119.474, 179.246, 234.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.710 - 3.000
R-factor	0.2156
Rwork	0.214
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v1d
RMSD bond length	0.010
RMSD bond angle	1.394
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.710	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.158	3.570
Rmeas	0.171	3.847
Rpim	0.065	1.426
Total number of observations	345622	32912
Number of reflections	50643	4598
<I/σ(I)>	8.7	0.8
Completeness [%]	99.9
Redundancy	6.8	7.2
CC(1/2)	0.996	0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.2 M Na/K Tartrate, 100 mM ADA pH 6.5