8F2T

Antibody Fab directed against SARS-CoV-2 Spike Protein Receptor Binding Domain (RBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	93.941, 60.868, 73.005
Unit cell angles	90.00, 98.75, 90.00

Refinement procedure

Resolution	28.340 - 2.120
R-factor	0.2078
Rwork	0.203
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ora
RMSD bond length	0.008
RMSD bond angle	1.017
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.340	2.160
High resolution limit [Å]	2.110	2.110
Rmerge		0.862
Number of reflections	23030	1154
<I/σ(I)>	9.9
Completeness [%]	97.3	65.4
Redundancy	6.59	5.5
CC(1/2)	0.920	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG 3350, 25 %, 0.1 M Tris, 0.2 M sodium chloride