8F2T
Antibody Fab directed against SARS-CoV-2 Spike Protein Receptor Binding Domain (RBD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.941, 60.868, 73.005 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 28.340 - 2.120 |
| R-factor | 0.2078 |
| Rwork | 0.203 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ora |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.340 | 2.160 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.862 | |
| Number of reflections | 23030 | 1154 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 97.3 | 65.4 |
| Redundancy | 6.59 | 5.5 |
| CC(1/2) | 0.920 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 3350, 25 %, 0.1 M Tris, 0.2 M sodium chloride |
