8F17
Structure of the STUB1 TPR domain in complex with H204, an all-D Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.18057 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.030, 72.710, 59.790 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 46.010 - 2.210 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q49 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.010 | 2.280 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.032 | 0.400 |
| Number of reflections | 16124 | |
| <I/σ(I)> | 16.9 | 2.3 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.15 M Sodium chloride, 0.1 M TRIS pH 8, 8% w/v PEG 6000 |
