8F14

Structure of the STUB1 TPR domain in complex with H201, an all-D Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.89685
Spacegroup name	C 2 2 21
Unit cell lengths	46.158, 90.115, 92.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.060 - 1.690
R-factor	0.1984
Rwork	0.197
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q49
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.330	1.720
High resolution limit [Å]	1.690	1.690
Rmerge	0.069	1.220
Number of reflections	21997	1103
<I/σ(I)>	20.2	2.2
Completeness [%]	100.0	100
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Lithium Sulfate, 0.1 M Bis-Tris pH 6.5, 25% w/v PEG 3350