8F0Y
Lipocalin-like Milk protein-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 |
Unit cell lengths | 32.672, 33.038, 38.640 |
Unit cell angles | 100.77, 99.68, 103.60 |
Refinement procedure
Resolution | 37.012 - 2.100 |
Rwork | 0.196 |
R-free | 0.25230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nyq |
RMSD bond length | 0.015 |
RMSD bond angle | 2.597 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.020 | 37.010 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.112 | 0.068 | 0.620 |
Rmeas | 0.158 | 0.096 | 0.877 |
Rpim | 0.112 | 0.068 | 0.620 |
Number of reflections | 8498 | 113 | 704 |
<I/σ(I)> | 3.5 | ||
Completeness [%] | 96.7 | 96.7 | 95.4 |
Redundancy | 2.6 | 2.6 | 2.6 |
CC(1/2) | 0.980 | 0.992 | 0.483 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | 0.2M Potassium Nitrate, pH 6.9, 20% PEG 3350 |