8F0N
Wobble Beetroot (A16U-U38G) dimer bound to DFHO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.012, 50.353, 94.584 |
Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
Resolution | 47.060 - 2.850 |
R-factor | 0.2004 |
Rwork | 0.194 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8eyu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.151 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.120 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.295 | 1.186 |
Rmeas | 0.321 | |
Rpim | 0.126 | |
Number of reflections | 6710 | 617 |
<I/σ(I)> | 2.5 | |
Completeness [%] | 99.9 | 90.47 |
Redundancy | 6.4 | 5.9 |
CC(1/2) | 0.989 | 0.482 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 2.4 M Ammonium sulfate, 5% 2-propanol |