8F0E

N-terminal WD40 domain of beta'-COPI subunit with four chains in the asymmetric unit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	49.115, 89.945, 138.324
Unit cell angles	90.00, 93.76, 90.00

Refinement procedure

Resolution	29.130 - 1.310
R-factor	0.1589
Rwork	0.157
R-free	0.19070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ewx
RMSD bond length	0.008
RMSD bond angle	1.096
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.370	1.330
High resolution limit [Å]	1.310	1.310
Number of reflections	286398	43255
<I/σ(I)>	6.5	1.4
Completeness [%]	99.7
Redundancy	4.5
CC(1/2)	0.998	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG 4000