8F0B

Lysozyme Anomalous Dataset at 240 K and 7.1 keV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	240
Detector technology	PIXEL
Collection date	2020-01-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.7462
Spacegroup name	P 43 21 2
Unit cell lengths	77.106, 77.106, 37.042
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.507 - 1.900
Rwork	0.137
R-free	0.19810
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.475
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.040	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.053	0.169
Rpim	0.018	0.067
Number of reflections	9246	590
<I/σ(I)>	35.4	12.6
Completeness [%]	100.0	100
Redundancy	15.8	12.5
CC(1/2)	0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	5 uL of 0.6 M Sodium Chloride in 100 mM Sodium Acetate buffer pH 4.5 and 25% ethylene glycol were mixed with 5 uL 100 mg/mL lysozyme in 100 mM Sodium Acetate.